Terminator

Part:BBa_K4577006:Design

Designed by: Ma Bourgeois   Group: iGEM23_Ionis-Paris   (2023-10-04)


T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To design a composite with the T7 promoter and terminator, it is important to ensure that the strain used for transformation contains T7 polymerase in its cell system. Otherwise the gene of interest will not be expressed.

Source

The use of the T7 system began in the 1980s, particularly in the laboratory of F. William Studier. Today, this system is used throughout the scientific world because of its simplicity.

References